The USNWC is home to over 40 miles of singletrack showcasing the preserved woodlands throughout the property and along the Catawba River. The trails offer varying levels of difficultly for guests of all ages and abilities. Mountain bikers, hikers, and runners alike share the trail system.

Trails are graded according to difficulty, and may be inaccessible during inclement weather, trail maintenance, or scheduled events (trail status is indicated at the bottom of any USNWC webpage). Helmets and mountain bikes designed for steep single-track terrain are required for all riders.